Journal: Bioactive Materials

Article Title: Pretreated exosomes by electrical stimulation accelerate bone regeneration

doi: 10.1016/j.bioactmat.2025.04.019

Figure Lengend Snippet: Histological staining of the distal femur defect site at 6-weeks post-injection. (A) Hematoxylin and eosin (H&E) staining, (B) Masson's trichrome staining, and (C) Immunohistochemical staining for OCN. D) The relative proportion of collagen from the quantitative analysis of Masson staining. E) Quantitative analysis results of IHC Results are shown as mean ± SD. ns: non-significant differences, ∗∗p < 0.01.

Article Snippet: They were then co-incubated with the primary antibody, specifically the OCN primary antibody from Beyotime, China.

Techniques: Staining, Injection, Immunohistochemical staining

Journal: Regenerative Biomaterials

Article Title: Combination of graphene oxide and platelet-rich plasma improves tendon–bone healing in a rabbit model of supraspinatus tendon reconstruction

doi: 10.1093/rb/rbab045

Figure Lengend Snippet: Primer sequences used for RT-PCR

Article Snippet: Samples were fixed with 4% paraformaldehyde for 30 min, treated with 0.5% Triton X-100 for 15 min and blocked with 2% BSA (Solarbio) for 40 min. After washing with PBS, the samples were stained against an OCN primary antibody (1:100; GeneTex, USA) or a Col II primary antibody (1:100; Proteintech, China) overnight.

Techniques: Sequencing

Journal: Regenerative Biomaterials

Article Title: Combination of graphene oxide and platelet-rich plasma improves tendon–bone healing in a rabbit model of supraspinatus tendon reconstruction

doi: 10.1093/rb/rbab045

Figure Lengend Snippet: ( A ) The expressions of genes related to osteogenic differentiation analyzed by qRT-PCR. ( B ) Immunofluorescence staining images of OCN expression at 14 d. ( C ) The expressions of genes related to chondrogenic differentiation analyzed by qRT-PCR. ( D ) Immunofluorescence staining images of col II expression at 21 d. Scale bar = 50 μm. * P < 0.05 and ** P < 0.01 vs PRP

Article Snippet: Samples were fixed with 4% paraformaldehyde for 30 min, treated with 0.5% Triton X-100 for 15 min and blocked with 2% BSA (Solarbio) for 40 min. After washing with PBS, the samples were stained against an OCN primary antibody (1:100; GeneTex, USA) or a Col II primary antibody (1:100; Proteintech, China) overnight.

Techniques: Quantitative RT-PCR, Immunofluorescence, Staining, Expressing

Journal: Stem Cell Research & Therapy

Article Title: A NOTCH1 / LSD1 / BMP2 co-regulatory network mediated by miR-137 negatively regulates osteogenesis of human adipose-derived stem cells

doi: 10.1186/s13287-021-02495-3

Figure Lengend Snippet: NOTCH1 inhibitor (tangeretin) reverses the effects of miR-137 knockdown on osteogenesis and downstream genes expression. a , b ALP staining ( a ) and quantification ( b ) of transfected hASCs after a 7-day culture in PM or OM (scale bar = 100 μm). c , d ARS staining ( c ) and quantification ( d ) of transfected hASCs after a 14-day culture in PM or OM. e Relative expression analyses of RUNX2 , ALP , and OCN by qRT-PCR in transfected hASCs on 3 days, 7 days, and 14 days. f Relative expression analyses of NOTCH1 , HES1 , LSD1 , BMP2 , and SMAD4 by qRT-PCR in transfected hASCs on 3 days, 7 days, and 14 days. g , h Western blotting ( g ) and band intensity analyses ( h ) of NOTCH1, HES1, LSD1, BMP2, SMAD4, and p-SMAD1/5 in transfected hASCs on 7 days. Data are shown as mean ± SD of three independent experiments performed in triplicate. * p < 0.05, ** p < 0.01, *** p < 0.001 versus respective NC group

Article Snippet: The rabbit anti-OCN primary antibodies diluted to 1:100 (Servicebio, Wuhan, Hubei, China; GB11233) were used for immunohistochemical (IHC) staining.

Techniques: Expressing, Staining, Transfection, Quantitative RT-PCR, Western Blot

Journal: Stem Cell Research & Therapy

Article Title: A NOTCH1 / LSD1 / BMP2 co-regulatory network mediated by miR-137 negatively regulates osteogenesis of human adipose-derived stem cells

doi: 10.1186/s13287-021-02495-3

Figure Lengend Snippet: HES1 knockdown promotes osteogenesis of hASCs in vitro. a , b ALP staining ( a ) and quantification ( b ) of transfected hASCs after a 7-day culture in PM or OM (scale bar = 100 μm). c , d ARS staining ( c ) and quantification ( d ) of transfected hASCs after a 14-day culture in PM or OM. e Relative expression analyses of HES1 , RUNX2 , ALP , and OCN by qRT-PCR in transfected hASCs on 3 days, 7 days, and 14 days. Data are shown as mean ± SD of three independent experiments performed in triplicate. * p < 0.05, ** p < 0.01, *** p < 0.001 versus respective NC group

Article Snippet: The rabbit anti-OCN primary antibodies diluted to 1:100 (Servicebio, Wuhan, Hubei, China; GB11233) were used for immunohistochemical (IHC) staining.

Techniques: In Vitro, Staining, Transfection, Expressing, Quantitative RT-PCR

Journal: Bioactive Materials

Article Title: A bone-targeted engineered exosome platform delivering siRNA to treat osteoporosis

doi: 10.1016/j.bioactmat.2021.09.015

Figure Lengend Snippet: BT-Exo-si Shn3 promote osteogenesis and angiogenesis in vitro . (A) Representative microscopic images showing mineralized matrix by ARS staining. (B) Quantitative analysis of ARS staining. (C) ALP activity of cells incubated with different nano-particles in normal NOIC medium and OIC medium on days 1, 4, 7 and 10. (D) Relative mRNA expression of osteogenesis-related genes (Runx2, OSX, OCN and OPN) after incubation with different nano-particles for 7 days in NOIC and OIC medium. (E) Bar graph data showing the fluorescence intensity of OSX and OCN. (F) Representative immunofluorescent images showing expression of osteogenic differentiation-related proteins (OSX and OCN) by MC3T3 cells after different treatments for 10 days. (G) Schematic illustration of pre-osteoblasts and EC coculture assays. (H) Expression level of VEGF, angiogenin and SLIT3 in the coculture system after different treatments. (I) Representative fluorescence images showing the tube formation of bEND.3 cells at 3 and 6 h. Scale bar = 100 μm. (J) Bar graph data showing the number of junctions in the tube network of bEND.3 cells after different treatments. (NS, no significant difference; *, p < 0.05; **, p < 0.01; ***, p < 0.001; #, p < 0.0001).

Article Snippet: The primary antibodies of OSX and OCN were then incubated with the secondary antibodies labelled with Cyanine 3 (Cy3, Servicebio, Wuhan, China).

Techniques: In Vitro, Staining, Activity Assay, Incubation, Expressing, Fluorescence